Zooming in on Diabetes Journal Club September 23, 2021

Zooming in on Diabetes Journal Club September 23, 2021

When:
September 23, 2021 @ 9:00 am – 10:00 am
2021-09-23T09:00:00-07:00
2021-09-23T10:00:00-07:00
Where:
ZOOM
Cost:
Free
Contact:
BC Diabetes Research Network

Zooming in on Diabetes Journal Club 

Thursday, September 23 at 09:00 (PST)

Presenter: Aurora Mattison, Graduate Student – PhD program, University of British Columbia (Vancouver)

Title of Paper:

Loss of Secretory Pathway Ca2+ ATPase (SPCA1) Impairs Insulin Secretion and Reduces Autophagy in the Pancreatic Islet

Abstract:

The β cell Golgi apparatus serves as a significant store of intracellular Ca2+ and an important site of proinsulin maturation. However, the contribution of Golgi Ca2+ to diabetes pathophysiology is unknown. The Golgi primarily utilizes the Secretory Pathway Ca2+ ATPase (SPCA1) to maintain intraluminal Ca2+ stores, and loss of SPCA1 has been linked to impaired Golgi function in other cell types. Here, we demonstrated that SPCA1 expression is decreased in islets from diabetic mice and human organ donors with type 2 diabetes, suggesting SPCA1 may impact diabetes development. INS-1 β cells lacking SPCA1 (SPCA1KO) showed reduced intraluminal Golgi Ca2+ levels, reduced glucose-stimulated insulin secretion (GSIS), and increased insulin content. Islets from SPCA1 haploinsufficient mice (SPCA1+/-) exhibited reduced GSIS, altered glucose-induced Ca2+ oscillations, and altered insulin granule maturation. Autophagy can regulate granule homeostasis, therefore we induced autophagy with Torin1 and found that SPCA1KO cells and SPCA1+/- islets had reduced levels of the autophagosome marker LC3-II. Furthermore, SPCA1KO LC3-II were unchanged after blocking autophagy initiation or autophagolysosome fusion and acidification. Thus, we concluded that β cell SPCA1 plays an important role in the maintenance of Golgi Ca2+ homeostasis and reduced Golgi Ca2+ impairs autophagy initiation and may impact insulin granule homeostasis.

URL link to publication source: https://www.biorxiv.org/content/10.1101/2021.08.30.458203v1.full

 

“Zooming in on Diabetes” is a virtual journal club and was launched in March 2020.

It is a joint initiative across the University of British Columbia campuses including the Life Sciences Centre and the BC Children’s Hospital.

This Journal Club is designed for trainees to develop skills in critical evaluation of recent articles in the scientific literature related to diabetes research.

The intended audience are academic research trainees and faculty involved in diabetes research in British Columbia, Canada.

The journal club is held on Tuesdays and Thursdays via ZOOM.

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