Calendar Overview

Zooming in on Diabetes Virtual Journal Club December 7
Dec 7 @ 9:00 am – 10:00 am


Thursday, December 7 at 9 am

Zooming in on Diabetes Journal Club


Dr Jia Zhao, Post-Doctoral Fellow, Kieffer Lab, Life Sciences Institute, University of British Columbia (Vancouver)


Enrichment of stem cell-derived pancreatic beta-like cells and controlled graft size through pharmacological removal of proliferating cells


Transplantation of limited human cadaveric islets into type 1 diabetic patients results in ∼35 months of insulin independence. Direct differentiation of stem cell-derived insulin-producing beta-like cells (sBCs) that can reverse diabetes in animal models effectively removes this shortage constraint, but uncontrolled graft growth remains a concern. Current protocols do not generate pure sBCs, but consist of only 20%-50% insulin-expressing cells with additional cell types present, some of which are proliferative. Here, we show the selective ablation of proliferative cells marked by SOX9 by simple pharmacological treatment in vitro. This treatment concomitantly enriches for sBCs by ∼1.7-fold. Treated sBC clusters show improved function in vitro and in vivo transplantation controls graft size. Overall, our study provides a convenient and effective approach to enrich for sBCs while minimizing the presence of unwanted proliferative cells and thus has important implications for current cell therapy approaches.

PMID: 37315522

URL link to publication source:

Subscribe To Our Newsletter
Subscribe to our email newsletter today to receive updates on the latest news and goings on.
No Thanks
Thanks for signing up. You must confirm your email address before we can send to you. Please check your email and follow the instructions.
We respect your privacy. Your information is safe and will never be shared.
Don't miss out. Subscribe today.