Zooming in on Diabetes Journal Club January 25, 2022
Zooming in on Diabetes January 25, 2022 at 12 pm (PST)
Marcus Woodley, Graduate Student – PhD program, Lynn Lab, BC Women’s and Children’s Hospital
Title: Ductal Ngn3-expressing progenitors contribute to adult β cell neogenesis in the pancreas
Citation:
C, G. et al. Ductal Ngn3-expressing progenitors contribute to adult β cell neogenesis in the pancreas. Cell stem cell (2021) doi:10.1016/J.STEM.2021.08.003.
Abstract:
Ductal cells have been proposed as a source of adult β cell neogenesis, but this has remained controversial. By combining lineage tracing, 3D imaging, and single-cell RNA sequencing (scRNA-seq) approaches, we show that ductal cells contribute to the β cell population over time. Lineage tracing using the Neurogenin3 (Ngn3)-CreERT line identified ductal cells expressing the endocrine master transcription factor Ngn3 that were positive for the δ cell marker somatostatin and occasionally co-expressed insulin. The number of hormone-expressing ductal cells was increased in Akita+/− diabetic mice, and ngn3 heterozygosity accelerated diabetes onset. scRNA-seq of Ngn3 lineage-traced islet cells indicated that duct-derived somatostatin-expressing cells, some of which retained expression of ductal markers, gave rise to β cells. This study identified Ngn3-expressing ductal cells as a source of adult β cell neogenesis in homeostasis and diabetes, suggesting that this mechanism, in addition to β cell proliferation, maintains the adult islet β cell population.
PMID: 34478642
URL link to publication source: https://www-sciencedirect-com.ezproxy.library.ubc.ca/science/article/pii/S1934590921003404?via%3Dihub
It is a joint initiative across the University of British Columbia campuses including the Life Sciences Centre and the BC Children’s Hospital.
This Journal Club is designed for trainees to develop skills in critical evaluation of recent articles in the scientific literature related to diabetes research.
The intended audience are academic research trainees and faculty involved in diabetes research in British Columbia, Canada.
The journal club is held on Tuesdays and Thursdays via ZOOM.
